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. Author manuscript; available in PMC: 2018 May 2.
Published in final edited form as: Structure. 2017 Apr 13;25(5):762–772.e4. doi: 10.1016/j.str.2017.03.008

Figure 3.

Figure 3

The WDR74 C-terminus is required for nucleolar localization (A) Multiple sequence alignment of the C-terminus of Nsa1/WDR74 from different homologues of Homo sapiens (Hsa), Mus musculus (Mmu), Xenopus tropicalis (Xtr), Danio rerio (Dre) Drosophila melanogaster (Dme), Schizosaccharomyces pombe (Spo), Candida albicans (Cal), and Saccharomyces cerevisiae (Sce). The alignment was done in Tcoffee and colored with Jalview. All of the lysine residues are highlighted in red. The final β strand from the WD40 domain is labeled. The sequence boundaries used for immunofluorescence are marked. (B) U2OS cells were transfected with plasmids harboring WDR74-GFP, WDR74ΔC (1–324)-GFP, WDR74ΔC (1–360)-GFP, or WDR74ΔN (320–385)-GFP, and then fixed and stained with DAPI (blue). The 20 μm scale bar is representative for all panels. (C) Western blot analysis of the interaction between NVL2 with various truncations of WDR74. See also Figure S6.