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Localization of WDR74. U2OS cells were transfected with a plasmid containing WDR74-GFP and treated with DMSO, actinomycin D, or rapamycin for 2 hours prior to fixing. Heat shock was induced for 3 hours at 43°C prior to fixing. The nucleus was visualized by staining with DAPI (blue) and the 20 μm scale bar is representative for all panels.
