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. Author manuscript; available in PMC: 2018 May 2.
Published in final edited form as: Structure. 2017 Apr 13;25(5):762–772.e4. doi: 10.1016/j.str.2017.03.008

Figure 6.

Figure 6

WDR74 binds the D1 domain of NVL2. (A) Schematic illustration of NVL2 and its truncations. The numbering is based on Mus musculus NVL2, which was used for the pull downs. (B) Western blot analysis of the domain mapping of the interaction between WDR74 and the individual domains of NVL2. The constructs of NVL2 used for the pull downs are depicted in figure 6A and contained the following residues FL (1–855), D1D2 (267–855), D1 (267–571), D1H7 (267–474), D2 (572–855) and NTD (1–266). (C) Western blot analysis of the mapping of the interaction between WDR74 and the D1-AAA domain of NVL2. The constructs of NVL2 used for the pull downs contained the following residues D1 (267–571), D1αβ (267–432), and D1α (432–571). (D) Surface representation of p97 taken from PDB ID 5C18. The NTD is shown in purple, D1αβ is shown in red, D1α is shown in pink and the D2 domain is shown in orange.