Figure 3.

Reactivation of aggregated Gfp-ssrA in the presence of ClpX. (A) Reactivation of aggGfp-ssrA (1.0 μM) was monitored as described in Materials and Methods in the absence (black circles) and presence (blue circles) of ClpX (0.3 μM), ATP (4 mM), and a regenerating system. Fluorescence emission (AU) was monitored for 30 min. The curves shown are representative of at least three replicates. (B) Reactivation of aggGfp-ssrA (1.0 μM) was monitored in the absence (black circles) or presence of ClpX (0.3 μM), ATP (4 mM) and a regenerating system (blue circles), ATPγS (2 mM) (orange circles), ADP (2 mM) (green circles), or no nucleotide (gray circles), where indicated. Fluorescence emission (AU) was monitored for 60 min. The curves shown are representative of at least three replicates.