Table 1.
E. coli strains and plasmids used in this study.
| Strain or plasmid | Genotype | Source, reference or Construction |
|---|---|---|
| STRAINS | ||
| BW25113 | F−, DE(araD-araB)567, lacZ4787(del)(::rrnB-3), LAM-, rph-1, DE(rhaD-rhaB)568, hsdR514 | Datsenko and Wanner, 2000 |
| JW0429 | F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), Δlon-725::kan, λ−, rph-1, Δ(rhaD-rhaB)568, hsdR514 | Baba et al., 2006 |
| JW0428 | F-, Δ(araD-araB)567,ΔlacZ4787(::rrnB-3), ΔclpX724::kan, λ−, rph-1, Δ(rhaD-rhaB)568, hsdR514 | Baba et al., 2006 |
| JW0427 | F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), ΔclpP723::kan, λ−, rph-1, Δ(rhaD-rhaB)568, hsdR514 | Baba et al., 2006 |
| JW2573 | F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), ΔclpB757::kan, λ−, rph-1, Δ(rhaD-rhaB)568, hsdR514 | Baba et al., 2006 |
| JW0866 | F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), ΔclpA783::kan, λ−, rph-1, Δ(rhaD-rhaB)568, hsdR514 | Baba et al., 2006 |
| JW3902 | F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), ΔhslU790::kan, λ−, rph-1, Δ(rhaD-rhaB)568, hsdR514 | Baba et al., 2006 |
| JW3903 | F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), ΔhslV720::kan, λ−, rph-1, Δ(rhaD-rhaB)568, hsdR514 | Baba et al., 2006 |
| JW0013 | F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), ΔdnaK734::kan, λ−, rph-1, Δ(rhaD-rhaB)568, hsdR514 | Baba et al., 2006 |
| JW0462 | F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), ΔhtpG757::kan, λ−, rph-1, Δ(rhaD-rhaB)568, hsdR514 | Baba et al., 2006 |
| JC0259 | MG1655 ΔclpX::kan | Camberg et al., 2011 |
| PLASMIDS | ||
| pET-ClpX | kan | Camberg et al., 2009 |
| pET-ClpP | kan | Maurizi et al., 1994 |
| pET-FtsZ | kan | Camberg et al., 2009 |
| pET-FtsZ(ΔC67) | kan | This study |
| pET-H6-Gfp(uv) | kan | This study |
| pGfp-ssrA | amp | Singh et al., 2000 |
| pClpX(E185Q) | amp | Camberg et al., 2011 |