Table 1.
Antibodies used in western blot assays and immunocytochemistry.
| Primary antibody | Species | Company | Dilution | Secondary antibody | Dilution | Company |
|---|---|---|---|---|---|---|
| Western blot | ||||||
| β-Actin | Mouse | Sigma–Aldrich | 1/40000 | Goat anti-mouse HRP | 1/5000 | Santa Cruz Biotech. |
| β-Tubulin | Mouse | Sigma-Aldrich | 1/1000 | Goat anti-mouse HRP | 1/5000 | Santa Cruz Biotech. |
| CD200 | Goat | R&D | 1/1000 | Mouse anti-goat HRP | 1/2000 | Sigma–Aldrich |
| CD200R1 | Goat | Santa Cruz Biotech. | 1/300 | Mouse anti-goat HRP | 1/2000 | Sigma–Aldrich |
| Immunohistochemistry | ||||||
| CD200 | Goat | R&D | 1/50 | Alexa 488 donkey anti-goat | 1/1000 | Invitrogen |
| CD200 | Rabbit | BIOSS | 1/50 | Alexa 488 donkey anti-rabbit | 1/1000 | Invitrogen |
| CD200R1 | Goat | R&D | 1/500 | Alexa 488 donkey anti-goat | 1/1000 | Invitrogen |
| GFAP | Rabbit | DAKO | 1/1000 | Alexa 546 donkey anti-rabbit Biotinylated goat anti-rabbit | 1/1000 1/500 |
Invitrogen Vector |
| Iba1 | Rabbit | Wako | 1/1000 | Alexa 546 donkey anti-rabbit Biotinylated goat anti-rabbit | 1/1000 1/500 |
Invitrogen Vector |
| MBP | Rat | Millipore | 1/1000 | Biotinylated goat anti-rat | 1/1000 | Invitrogen |
| NeuN | Mouse | Millipore | 1/500 | Alexa 546 donkey anti-rabbit | 1/1000 | Invitrogen |
CD200R1, CD200 receptor 1; GFAP, glial fibrillary acidic protein; Iba1, ionized calcium binding adaptor molecule 1; MBP, myelin basic protein; NeuN, Neuronal nuclei.
Companies: Sigma–Aldrich, St. Louis, MO, USA; R&D Systems Inc., Minneapolis, MN, USA; Santa Cruz Biotechnology, Inc., Dallas, Texas, USA; BD Pharmingen: BD Bioscencies, San José, CA, USA; Abcam plc, Cambridge, United Kingdom; Bioss antibodies, Woburn Massachusetts, USA; Dako, Carpinteria, CA, USA; Wako Pure Chemical Industries Ltd, Tokyo, Japan; Millipore, Bedford, MA, USA; Invitrogen Molecular Probes, Eugene, OR, USA; Vector Laboratories Inc., Burlingame, CA, USA.