. 2017 May 4;8:723. doi: 10.3389/fmicb.2017.00723

Table 1.

Proteins identified in the immunoreactive bands from the two Brachyspira species.

Band id^a	Sample	Specific^b	Observed Mass (kDa)		Protein identified
			12%	7.5%	Accession^c	Name	Theor. Mass (kDa)
2	OLA9	X	86	76	D8ICZ7	Elongation factor G	75.8
3	OLA9		83		D8IDC2	Uncharacterized protein	62
4	ATCCBP	X	81	78	D8ICZ7	Elongation factor G	75.8
5^*	OLA9	X	78	72	J9UT37	Putative polymerase	72.3
					D8IEM8/A0A0G4K4U5	Chaperone protein HtpG	73.9
					D8IBS0	Chaperone protein DnaK	67.6
6	ATCCBP		77		D8IDC2	Uncharacterized protein	62
7	OLA9		75		D8IDC2	Uncharacterized protein	62.1
9	V1		73		G0EJY7	Uncharacterized protein	65.4
10^*	ATCCBP	X	72	70	D8IEM8	Chaperone protein HtpG	73.9
					J9UT37	Putative polymerase	72.3
					D8ICG5	Uncharacterized protein	85
12	V1		72		C0R0R7	Putative treponemal membrane protein	63.9
13	OLA9	X	72	68	D8IE58	Aspartyl-tRNA synthase	67.5
15	ATCCBP		69		D8IDC2	Uncharacterized protein	62.1
16	ATCCBP	X	69		D8IFS5	Phosphoenol pyruvate carboxykinase	67.5
17	ATCCBP	X	69	70	D8IE58	Aspartyl-tRNA synthase	67.5
18	V1	X	68		C0R0E5	Phosphoenol pyruvate carboxykinase	67.5
19	OLA9	X	65		D8I9T6	Biotin lipoyl	65
20	OLA9		64	61	D8IB78	60 kDa chaperonin	58.1
22	ATCCBP	X	63		D8I9T6	Biotin lipoyl	65.0
24	ATCCBP		62	61	D8IB78	60 kDa chaperonin	58.1
25	V1		61		C0QWH4	60 kDa chaperonin	58.2
26^*	OLA9	X	58	53	J9USS2	60 kDa chaperonin	58.1
					D8IAM3	Amidohydrolase 3	60.6
					J9UU81	2-isopropylmalate synthase	54.4
					D8IF78	Outer membrane efflux protein	54.7
					D8IDP7	Trigger factor, C-terminal domain protein	50.6
27	OLA9	X	57		D8IBK7	TmpB outer membrane protein	42.5
28	ATCCBP	X	56	55	K0JIZ9	Carboxyl terminal protease	54.9
29^*	ATCCBP	X	54		D8IBK7	TmpB outer membrane protein	42.4
31	ATCCBP	X	51	50	D8IBH9	ATP synthase subunit beta	45.6
32	ATCCBP	X^*	50		D8IET2	Enolase	47
36	V1	X	45		C0QW84	Uncharacterized protein	38.6
37	OLA9		44		Q9FA06	Putative elongation factor Tu	16.3
					D8ICZ6	Elongation factor Tu	44.8
38	ATCCBP		44		Q9FA06	Putative elongation factor Tu	16.3
					D8ICZ6	Elongation factor Tu	44.8
39	ATCCBP	X	44		D8ICG3	Uncharacterized protein	38.9
40	OLA9	X	43	42	D8IBY6	FlaA	35.7
41	V1		43		C0QVZ4	Elongation factor Tu	44.7
					Q9FA06	Putative elongation factor Tu	16.3
42	V1		42		C0QXS8	NADH oxidase	50.5
					C0QVZ4	Elongation factor Tu	44.7
43	V1		42		C0R0T5	FlaA	24.5
					P32520	FlaA1	36
44	ATCCBP	X	41		D8IBY6	FlaA	35.7
45	OLA9	X	40	38	D8ICU0	VspD	42.7
46^*	ATCCBP	X	38	38	D8ICU0	VspD	42.7
					J9TU32	Ribonucleotide-diphosphate reductase subunit beta	41.1
					D8IEW7	Mannose-1-phosphate guanylyltransferase	40.5
					J9URY6	2-oxoacid:ferredoxin oxidoreductase subunit alpha-like protein	82.8
					J9UBH8	ATP-dependent 6-phosphofructokinase	34.8
					D8ICA2	Toxic anion resistance family protein	40.9
					D8I9T4	Uncharacterized protein	39.9
					D8ICR1	Pyruvate oxireductase	35.5
47	OLA9		36		D8IAP2	Flagellin FlaB2	32
48	V1	X	35		C0R1L9	UDP-glucose 4 epimerase	36
					C0QYC2	Galactose-glucose binding protein	38.2
49	ATCCBP		35		D8IAP2	Flagellin FlaB2	32
50	V1		35		Q26501	FlaB1	32
51^*	V1	X	35		A0A0H0W3D6	Enolase	47
					Q26501	Flagellar protein FlaB1	32
					A0A0HOUMF4/G0EJK5	Fructose-bisphosphate aldolase	35.4/35.4
					C0QYC2	Methyl-galactoside ABC transporter substrate-binding protein	38.2
					C0QZV6	Pseudouridine-5'-phosphate glycosidase	33.9
52	OLA9	X^*	33		D8ICA8	Putative FlaA	26.7
53	OLA9		32		K0JHQ4	Flagellin FlaB2	31.3
54	V1		31		P80160	Flagellin FlaB2	31.1
55	ATCCBP		31		K0JHQ4	Flagellin FlaB2	31.3
56	OLA9		29		K0JLS4	Flagellin FlaB3	30
57	V1	X^*	29		C0QV52	Enolase	47
					Q9F0F6	Flagellin FlaB3	30.4
58	ATCCBP		29		D8IDG1	Flagellin FlaB3	26.6
59	V1		29		Q9F0F6	Flagellin FlaB3	30.4
61	V1	X	26		C0QWY9	Putative FlaAL	24.7
62	ATCCBP	X	24		D8ICA7	FlaA-2	24

Complete identification data is available in Supplementary Table S1 (MALDI TOF/TOF) and Supplementary Tables S3–S9 (LC-MS/MS). Specific, indicates immunoreactivity is only detected in challenged animals. Observed mass, correspond to the experimental mass calculated from the gel bands. ATCCBP, ATCC 51139 (P43/6/78) B. pilosicoli strain.

All identifications by MALDI TOF/TOF except those indicated with an asterisk which were identified by LC-MS/MS.

Specific bands with X^* have been detected in a control serum with an intensity of one.

Group head accession, full data in Supplementary Tables S1, S2.