Table 7.
Characterization of 23 novel microsatellite loci for Sudan grass.
| Gene ID | Primer no. | Forward primer(5′–3′) | Reverse primer (5′–3′) | Tm | Product size(bp) | Repeats | PIC | I | H | PPB% |
|---|---|---|---|---|---|---|---|---|---|---|
| c25684_g1 | 1 | GGTAGGAGACGGAGGTGAGT | GTCCTCCGATCTGCTAAGCC | 60 | 140 | (GCG)6 | 0.7608333 | 0.4577 | 0.3537 | 100.00 |
| c24947_g1 | 2 | GTTTGCTTGTCCGCTAAGGC | TTGTCGTCGTCCGTTGTAGG | 60 | 197 | (ACC)6 | 0.6325 | 0.6567 | 0.4902 | 100.00 |
| c32362_g4 | 3 | GCAACGGCAAGCAGAGTATG | TTTGCATGCCACAGACTTGC | 59 | 255 | (GCA)7 | 0.4908333 | 0.5555 | 0.4925 | 66.67 |
| c18739_g1 | 4 | ACAGCTAGTTTGGGTGTCCG | CATGGCCGAGGTCAACTCTT | 59 | 140 | (GCT)6 | 0.6357143 | 0.5622 | 0.4607 | 100.00 |
| c30194_g1 | 5 | TCACGCACTGCAGGGTTAAT | ACACGCGTATCAGTGTTCGT | 59 | 134 | (A)10 | 0.7336364 | 0.6645 | 0.1948 | 100.00 |
| c23844_g2 | 6 | CCGCCAAAGAAGACAAAGGC | GTCCAGCTCCATCCTGAACC | 60 | 218 | (CGA)6 | 0.7140625 | 0.4848 | 0.4030 | 100.00 |
| c10226_g1 | 7 | GTGACACGGATCTCACGGAG | GGTGTGGTGAAGAGGTCTGG | 60 | 192 | (TTG)5 | 0.3033333 | 0.6109 | 0.5281 | 33.33 |
| c29788_g1 | 8 | GCGGGTGTTGATGATGCTTG | GCCAAAGCAAGAACGACAGG | 60 | 104 | (GCC)5 | 0.3442857 | 0.5156 | 0.5132 | 57.14 |
| c25493_g1 | 9 | TGCTGAAACTTTGGAATGGCA | CCCCTTGCATGTTCAAGTGC | 58 | 154 | (T)11 | 0.5883333 | 0.6851 | 0.4850 | 83.33 |
| c35250_g7 | 10 | CAATTTTGCCCTCCTTGCCC | CTTCCTCATCTCCTCCGCAC | 60 | 242 | (T)11 | 0.39075 | 0.5207 | 0.5045 | 50.00 |
| c28737_g2 | 11 | AAGATCAAGAAGCCCCTGCC | ATCTGGTGGTACCTCGGGAA | 60 | 143 | (GCC)5 | 0.7382143 | 0.5887 | 0.3875 | 85.71 |
| c22092_g1 | 12 | TGTGACTTTGAGCTCCGTCA | TGCTACCTGAACTCCCCCTT | 59 | 229 | (T)11 | 0.3525 | 0.5943 | 0.4448 | 66.67 |
| c39996_g1 | 13 | TCCGAAATCCTTGTCCCTCT | TCGCCATCGATCAACTCTCG | 58 | 121 | (CAC)5 | 0.453 | 0.6227 | 0.5120 | 80.00 |
| c33403_g4 | 14 | GAGCAGAGGAACCATGGCTT | TCGTCACGCAACAACTGTTC | 60 | 200 | (A)10 | 0.41875 | 0.5554 | 0.5169 | 50.00 |
| c31134_g1 | 15 | AGGTCAGACGCCAAACTGTC | ATCAGCACGTACTTGGTCGG | 60 | 234 | (TTC)5 | 0.1642857 | 0.4127 | 0.4668 | 28.57 |
| c32157_g1 | 16 | CCACATCCGTTCACCCTTCA | GGGTTTGGTTGCTCCGTTTC | 59 | 206 | (CT)6 | 0.57 | 0.5004 | 0.4693 | 100.00 |
| c32633_g2 | 17 | CTCTCTCCCACTCTCTGCCT | AGGGAAGTGCATGCAAGCTA | 60 | 163 | (TCG)5 | 0.715 | 0.3902 | 0.3791 | 100.00 |
| c30552_g1 | 18 | TCCTATGCGCACCACCAATT | GTGACATGATCCCTGGCCC | 60 | 248 | (GC)6 | 0.6275 | 0.5088 | 0.4187 | 66.67 |
| c40055_g1 | 19 | GAGCATCGAGGAGCGTCTC | CTTCTGCGCTCGAAATGGAG | 60 | 183 | (C)11 | 0.23625 | 0.3183 | 0.4424 | 100.00 |
| c23435_g1 | 20 | GGGGCAAAACTGCTGTTGTT | CCCACTTCCTGCTCAACCAG | 59 | 157 | (CTG)5 | 0.66375 | 0.6276 | 0.3963 | 75.00 |
| c32482_g1 | 21 | CGTAATTTGGGCCGCCAATC | CGAACGAATCGCTGAGGTTT | 60 | 275 | (A)10 | 0.626875 | 0.6272 | 0.4561 | 75.00 |
| c17048_g1 | 22 | CATAGTGCCGTAGCCCGTAG | CGGACGACTCTTGGACCAAA | 60 | 280 | (GGC)5 | 0.2615 | 0.5579 | 0.4745 | 80.00 |
| c24737_g1 | 23 | GCAAGATGATATATGGAGTACGGC | TCCCCTTCCTAGAGCTGCTT | 59 | 140 | (A)10 | 0.8304167 | 0.6704 | 0.3114 | 100.00 |
Tm, optimal annealing temperature (°C); PIC, average polymorphism information content; I, Shannon's information index of diversity; H, Nei's gene diversity; and PPB, percentage of polymorphic bands.