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. 2017 May 4;11:135. doi: 10.3389/fncel.2017.00135

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Copyright © 2017 Gimenez-Molina, Villanueva, Nanclares, Lopez-Font, Viniegra, Francés, Gandia, Gil and Gutiérrez.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Distribution of F-actin in chromaffin cells from adrenal medulla slices. Sequential confocal images of cells in slices of adrenomedullary tissue labeled with rhodamine–phalloidin (red) and anti-SNAP-25 (green) (A) showing a broader cytoplasmic distribution of F-actin, as confirmed by the intensity study of the central image (B), and the spatial plane analysis (C). (D) The individual fluorescence profiles were averaged to obtain the distribution of F-actin fluorescence (red line) relative to the plasma membrane position (green line) of cells in the adrenal medulla (n = 16 cells, two different tissue preparations). Bars in (A,B) represent 1 μm.