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. 2017 May 4;11:135. doi: 10.3389/fncel.2017.00135

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Copyright © 2017 Gimenez-Molina, Villanueva, Nanclares, Lopez-Font, Viniegra, Francés, Gandia, Gil and Gutiérrez.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Chromaffin granule distribution studied by EM in isolated cells and cells present in the adrenal medulla. EM micrographs corresponding to individual cultured cells (A) and cells present in the adrenomedullary slices (B). These images were used to calculate the granule position with respect to the cell membrane, generating the granule distributions depicted for the cultured cell sampled (C) and the cell in the tissue (E). Vesicle distributions were used to obtain the averaged granule distributions for cultured cells (D, n = 10 cells, 308 vesicles) and cells in the adrenal tissue (F, n = 10 cells, 302 vesicles). The curves in (D,F) correspond to the best fit to a binomial distribution. Bars in (A,B) represent 2 and 5 μm, respectively.