FIGURE 7.

Secretory response from populations of isolated packed cells and pieces of adrenomedullary tissues. Catecholamine release from populations of chromaffin cells measured amperometrically upon stimulation of the cells with 5 s pulses with a Krebs-HEPES solution containing 100 μM ACh. (A) Show representative amperometric recordings obtained from packed isolated cells or from adrenal tissue pieces as indicated in methods. (B) Shows the averaged catecholamine release from isolated cells versus the release obtained in adrenal tissue. The data are the means ± SEM of nine experiments from three different preparations. (C) The averaged catecholamine release has been normalized to the maximal release in order to show that there are no changes in the onset kinetics and only a small change in the offset kinetics when the responses obtained in isolated cells were compared with those from adrenal tissue.