FIG 3 .

RVFV-derived small RNAs are present in three different vector mosquito species. The small-RNA fraction from RVFV MP12-infected A. aegypti, A. vexans, and C. quinquefasciatus whole mosquito bodies was isolated 14 days p.i. (A. aegypti and C. quinquefasciatus) or 15 days p.i. (A. vexans) and sequenced (A to C). The graphs represent the small-RNA reads mapping to the RVFV MP12 genomic and antigenomic sequences. Read numbers per length were plotted for A. aegypti (A), A. vexans (B), and C. quinquefasciatus (C) against the corresponding read length (in nucleotides) on a linear scale. (D to F) Alignment of the 21-nt small-RNA reads mapping to the RVFV MP12 sequences of the three RNA segments for all mosquito species as described for RVFV-derived small-RNA reads in Aag2 cells (Fig. 1C). The alignments for A. aegypti (D), A. vexans (E), and C. quinquefasciatus (F) are shown.