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. 2016 Oct 31;36(16):2228–2242. doi: 10.1038/onc.2016.371

Figure 7.

Figure 7

ANGPTL4-induced MMP-1 is dependent on c-Jun expression. (a) The MMP-1 promoter containing wild-type (Wt) and AP-1 binding site mutants (mt1 and mt2) was constructed. (b) TU183 cells were co-transfected with 0.5 μg MMP-1 promoter and 20 ng renilla luciferase construct, and then treated with CM collected from EGF-treated cells (i). TU183 cells were co-transfected with 0.5 μg MMP-1 promoter and 20 ng renilla luciferase construct and 1 μg flANGPTL4-expressing vector (ii). The firefly and renilla luciferase activities were then determined and normalized. Values are the mean±s.e.m. (c) TU183 cells were co-transfected with 0.5 μg MMP-1 promoter, 20 ng renilla luciferase construct, 50 nM c-Jun siRNA oligonucleotides, scramble siRNA (Sc) (i) and myc-c-Jun-expressing vector (ii) by lipofection and then with or without 250 ng/ml cANGPTL4 for 18 h. The firefly and renilla luciferase activities were then determined and normalized. Values are the mean±s.e.m. The expression levels of c-Jun, β-actin, α-tubulin and phosphorylated c-Junser73 were analysed by western blotting. ***P<0.005.