Fig 4.

Ormdl3 KO^Mer mice have diminished cellular stress responses to Alternaria. A, Schematic showing major components of the UPR. B, Western blot showing p-eIF2α. Specific quantitative PCR analyses were performed for Atf4(C), Ddit3(D), and total Xbp1(E). F, PCR of total and spliced Xbp1. G,Edem1 quantitative PCR. H, IL-6 determined by ELISA. I, Western blot showing SERCA2b. Uric acid (J), LDH (K), and albumin (L) levels in BALF. Alt, Alternaria; Ddit3, DNA-damage-inducible transcript 3; Edem1, ER degradation enhancer, mannosidase alpha-like 1. Data were collected from 2 individually performed experiments. N = 8-16 mice per group. Box and whisker plots depict the median and interquartile range and minimum and maximum values. *P < .05, **P < .005, and ***P < .0005. For analysis of gene expression, Mann-Whitney statistical tests were performed between groups where a greater than 2-fold induction of median values was observed.