Fig. 4.

Effect of HSYA on NF-κB activation in rat spinal cord. a, b Representative result from EMSA shows DNA-binding activity of NF-κB was at very low levels in the sham group but significantly increased in the control group, but significantly decreased after HSYA treatment. c, d The activity of NF-κB by Western blot, results of NF-κB nucleus importation was unanimous as that of EMSA, but no significant differences were observed on NF-κB in cytoplasm. This figure is representative of three experiments performed on different experimental days. (*P < 0.05 vs. sham group; ●P > 0.05 vs. control group; P < 0.05 vs. sham group; **P < 0.01 vs. sham group; ^▲ P < 0.05 vs. sham group; P < 0.01 vs. control group)