Fig. 5.

Proteins differentially expressed by HTR-8/SVneo cells cultured under serum-depleted conditions. a HTR-8/SVneo cells were grown in medium with 10 or 0 % FBS for 24 h (in triplicate). Total protein extracts (0.50 mg) were separated by 18 cm 2DE gels as previously described (n = 3). The protein spots were analyzed via LC ESI-Q/TOF and identified using bioinformatics analysis. MW – molecular weight marker (RPN5800, GE). b Effect of serum depletion on the expression of some trophoblast proteins. Statistical analysis of 2DE protein spots identified by MS/MS in HTR-8/SVneo proteomes either with 10 % FBS or without FBS (n = 3). Kolmogorov-Smirnov test (D value): 0.10, D > 0.642 (a, D = 1.0; b, D = 0.67)