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. 2017 Apr 4;22:7. doi: 10.1186/s11658-017-0036-2

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Effect of ABCC6 knockdown on GSH/GSSG ratio and ROS generation. a – The GSH/GSSG ratio was determined using the glutathione fluorometric assay, as described in the Materials and Methods section. The results are reported as means ± SD of three independent experiments performed in triplicate. b – ROS production was detected via FACS using a DCFH-DA fluorescent probe as described in the Materials and Methods section. Scr-shRNA: HepG2 cells stably transfected with scrambled shRNA; ABCC6-shRNA: HepG2 cells stably transfected with ABCC6-shRNA. The values are the means ± SD of three replicates from three independent experiments. **p < 0.01. Unpaired data were assessed for statistical significance using Student’s t test