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. 2016 Jul 28;21:8. doi: 10.1186/s11658-016-0009-x

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Morphology and immunofluorescence characterization of MDSCs. a Morphology of MDSCs during differentiation for 0, 1, and 3 days (MDSC-P, MDSC-D1, and MDSC-D3, respectively); b Immunofluorescence detection of MHC in MDSCs during differentiation at MDSC-P, MDSC-D1 and MDSC-D3; c Immunofluorescence detection of desmin in MDSCs during differentiation at MDSC-P, MDSC-D1 and MDSC-D3. d Expression of MRF during MDSC differentiation. The RT-qPCR analysis of PAX3, MYOD, MYOG, MYF5, and MYF6 genes in MDSC-D1 and MDSC-D3 compared to MDSC-P. Error bar indicates standard error of mean of triplicate samples. *p < 0.05,**p < 0.01