Table 1.
Summary of small RNA sequencing data
| Type | MDSC-P | MDSC-D1 | MDSC-D3 | |||
|---|---|---|---|---|---|---|
| Count | % | Count | % | Count | % | |
| total_reads | 6000000 | 6000000 | 6000000 | |||
| high_quality | 5984373 | 100 % | 5984335 | 100 % | 5984569 | 100 % |
| 3'adaptor_null | 2916 | 0.05 % | 2967 | 0.05 % | 2901 | 0.05 % |
| insert_null | 3115 | 0.05 % | 1252 | 0.02 % | 1408 | 0.02 % |
| 5'adaptor_contaminants | 86464 | 1.44 % | 54566 | 0.91 % | 39651 | 0.66 % |
| smaller_than_18nt | 20713 | 0.35 % | 3297 | 0.06 % | 4615 | 0.08 % |
| polyA | 110 | 0.00 % | 65 | 0.00 % | 31 | 0.00 % |
| clean_reads | 5871055 | 98.11 % | 5922188 | 98.96 % | 5935963 | 99.19 % |
Note: total_reads: total sequenced reads, which is required to be greater than 5 M in general; high_quality: number of high quality reads with no N, no more than 4 bases whose quality score was lower than 10 and no more than 6 bases whose quality score was lower than 13; 3'adaptor_null: number of reads with no 3'adaptor; insert_null: number of reads with no insertion; 5'adaptor_contaminants: number of 5'contaminants; smaller_than_18nt: number of reads less than 18 nt generally. Small RNA tags were between 18 and 30 nt long, so too short tags should be removed from data for further analysis; polyA: number of reads with polyA; clean_reads: number of clean reads after adaptors and contaminants were removed, which were used in the following analysis