Figure 5.

GRIK5, ALDH5A1, and GABRA3 Expression Was Analyzed by Real-Time PCR/Western Blot and Hypothesis of an Ocular Disease Mechanism

(A) The mRNA expression levels of GRIK5, ALDH5A1, and GABRA3 in HLE-m-miR-184 versus HLE-NC. (B) The mRNA expression levels of GRIK5, ALDH5A1, and GABRA3 in HLE-miR-184 versus HLE-NC. (C) The mRNA expression levels of GRIK5, ALDH5A1, and GABRA3 in HLE-m-miR-184 versus HLE-miR-184. Expression of GRIK5, ALDH5A1, and GABRA3 was determined by real-time PCR after miR-184, m-miR-184, or NC transfecting HLE for 24 hr. The error bars represent the data in triplicates. Results are displayed as mean ± SEM (**p < 0.01 and *p < 0.05). (D) ALDH5A1, GABRA3, and DGRIK5 expression levels were determined by western blot after HLE cells were transfected with m-miR-184, miR-184, or NC for 48 hr. GAPDH was used as an internal control. (E) m-miR-184 exposure affects the alanine, aspartate, and glutamate metabolism pathway by the downregulation of ALDH5A1 and GABRA3, contributing to dysfunction of the citrate cycle.