Figure 3.

Quantitative Evaluation of Exon 7 Inclusion after tcDNA TCI7 Treatment in SMA Mice

Detection of the inclusion of exon 7 of SMN2 mRNA by qPCR in heart and brain of SMA type III mice. The primers used for amplification are located on exon 6 and 7 and normalized with GAPDH. Un, untreated mice; 4wk, mice treated for 4 weeks with 200 mg/kg per week by subcutaneous injection of TCI7; 12wk, mice treated for 12 weeks with 200 mg/kg per week by subcutaneous injection of TCI7; n = 3 per group.