Figure 1.

Expansion of Purified iPSC-Derived Myogenic Progenitors

(A) I, Scheme for differentiation of iPSCs into myogenic progenitors and FACS purification; II, Expansion of purified myogenic progenitors. The expansion medium is indicated. The fold expansion is indicated. (B) Immunofluorescent staining for Pax7 (in red) in selected areas from a culture dish derived from non-purified myogenic progenitors following the 35-day differentiation protocol. The nuclei were stained with Hoechst (blue). (C) Linear proliferation curves for all four iPSC-derived myogenic progenitor lines during expansion. The average R² shown was calculated for all data points of the four lines and indicates high concordance between the four lines. (D) Karyotype analysis after expansion of purified myogenic progenitors at day 35 (a representative example of ten nuclei). (E) Immunofluorescent analysis of MyoD in FACS purified and expanded myogenic progenitors. Myogenic progenitors were expanded in proliferation medium and stained at the start of expansion and after expansion to 10¹² cells. Representative pictures are shown in the image.