Figure 6.

Rpd3L histone deacetylase recruitment at LTV1 promoter. Indicated strains expressing the Sds3-13xMyc-tagged protein were grown exponentially in YPD at 30°C and shifted to YP medium without glucose. Samples were collected before shift and after 10, 20 or 30 min for ChIP-qPCR analysis of Sds3-13xMyc association with the LTV1 promoter. Fold-enrichment relative to the control ADH4 promoter is represented in either wild type (WT, black bars) and promoter-mutated LTV1 gene at Abf1 binding site (LTV1 Amut, white dotted bars). Data are presented as mean ± SEM of four independent replicates. For wild type, a one-way ANOVA test was used to compare the means of measurements at each time point respect to WT t0; for the enrichment in the mutant a one-way ANOVA test was used to compare the means of measurements of mutants with respect to WT at each time point. *P < 0.05, **P < 0.01, ***P < 0.001 using a Tukey post-hoc test.