Table 1.

Primers Used for Introduction of the MCT8 Mutations

Mutation	Location	Reference	Primers		Tm (°C)
G221R	TMD 2	29	Forward	5′-CAAGCAGCATGGGTCAGAGCCCTCGCGATG-3′	81.8
			Reverse	5′-CATCGCGAGGGCTCTGACCCATGCTGCTTG-3′
InsV236	TMD 2	30	Forward	5′-CTTCTGTTCTCCCATTGTGGTGAGTATATTCACTGACCG-3′	80.8
			Reverse	5′-CGGTCAGTGAATATACTCACCACAATGGGAGAACAGAAG-3′
G282C	TMD 4	31	Forward	5′-GATTCTCTTTGGTTGTTGCTGTTCCTTCGCCTTTC-3′	78
			Reverse	5′-GAAAGGCGAAGGAACAGCAACAACCAAAGAGAATC-3′
P321L	TMD 5	29	Forward	5′-CATTTTCTCCATGTCCTTCCTCTTCCTCATCAGAATGCTG-3′	80.1
			Reverse	5′-CAGCATTCTGATGAGGAAGAGGAAGGACATGGAGAAAATG-3′
D453V	TMD8/ICL 4	33	Forward	5′-CAGGCCACATCAGTGTCTCCATCCCTGGAC-3′	80.6
			Reverse	5′-GTCCAGGGATGGAGACACTGATGTGGCCTG-3′
P537L	TMD 11	32	Forward	5′-CCAATGATTGCTGGGCTCCCCATTGCAGGCCTAC-3′	82.8
			Reverse	5′-GTAGGCCTGCAATGGGGAGCCCAGCAATCATTGG-3′
G558D	TMD 12	7	Forward	5′-GCCTTCTACTTTGCCGATGTGCCCCCCATCATCG-3′	82.8
			Reverse	5′-CGATGATGGGGGGCACATCGGCAAAGTAGAAGGC-3′

Tm, melting temperature; TMD, transmembrane domain; ICL, intracellular loop.