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. 2017 Feb 13;45(8):4550–4563. doi: 10.1093/nar/gkx096

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© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 7. — Analysis of DNA synthesis with CTF18-RFC–Polε–PCNA. Product DNA profiles after electrophoresis in alkaline agarose gels are indicated together with DNA size markers (right). (A) Holoenzyme assay was done with indicated combinations of 200 fmol Polε, 600 fmol CTF18-RFC, 2 pmol PCNA and 6 pmol RPA. Lower amounts of CTF18-RFC (400 or 200 fmol) were used in lanes 6 and 7. (B) Titration of Polε (+, ++, +++ for 147, 293, 440 fmol, respectively; lanes 2–8) or Polδ (+, ++, +++ for 53, 107, 160 fmol, respectively; lanes 9–15) in the holoenzyme assay with 600 fmols RFC (lanes 3–5, 10–12) or CTF18-RFC (lanes 6–8, 13–15) or without (lanes 2 and 9) in the presence of 6 pmol RPA and 2 pmol PCNA.