Table 1. Oligonucleotides used in this study.
Name | Sequence (5΄–3΄) |
---|---|
BTN5 | Biotin-tgaggttcagcaaggtgatgctttagatttttcatttgctgctggctctcagcgtggca-ctgttgcaggcggtgttaatactgaccgcct |
BTN3 | tgaggttcagcaaggtgatgctttagatttttcatttgctgctggctctcagcgtggcactgttgc-aggcggtgttaatactgaccgcct-Biotin |
BTN32 | aaaaatctaaagcatcaccttgctgaacctca |
BTN30 | cagtgccacgctgagagccagcagcaaatg |
BTN28 | aggcggtcagtattaacaccgcctgcaa |
TEMP90-R | aggcggtcagtattaacaccgcctgcaacagtgccacgctgagagccagcagcaaatgaaaaatct-aaagcatcaccttgctgaacctca |
TEMP90-R neo | gaaaaatctaaagcatcaccttgctgaacctcaaggcggtcagtattaacaccgcctgcaacagtg-ccacgctgagagccagcagcaaat |
RF64 | aggcggtcagtattaacaccgcctgcaacagtgccacgctgagagccagcagcaaatgaaaaat |
RF30 | tgaggttcagcaaggtgatgctttagattt |
RF46 | tgaggttcagcaaggtgatgctttagatttttcatttgctgctggc |
RF41 | tgaggttcagcaaggtgatgctttagatttttcatttgctg |
RF36 | tgaggttcagcaaggtgatgctttagatttttcatt |
RF31 | tgaggttcagcaaggtgatgctttagatttt |
RF26 | tgaggttcagcaaggtgatgctttag |
RF21 | tgaggttcagcaaggtgatgc |
BTN5, BTN3 and their annealing primers (BTN32, 30 and 28) have been described previously (33).