Figure 3.

Rewiring terpenoid metabolic pathways for heterologous production of lycopene. (A) Genetic constructs in engineered lycopene-producing P. pastoris strain. The bacterial genes crtE, crtBand crtI were placed under the control of constitutive promoters to enable lycopene production. The rewiring promoter and CDS library and lycopene pathway were cloned into the his4 locus in a single vector. (B) Mean production of lycopene in 40 ml cultures. Assayed at 48 and 72 h post inoculation (N = 3,±SD). t-test: *P < 0.05, **P< 0.01, ***P < 0.0001.