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. 2017 Jan 4;45(8):e62. doi: 10.1093/nar/gkw1329

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© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 4. — Real-time PCR genotyping plots. X-axis show value of FAM probe signal (transgene specific probe). Y-axis show value of HEX probe signal (wild-type specific probe). RFU: relative fluorescence units. Each data point represents one animal. C_q values are means of values from two technical replicates. For each plot the tested genotypes are indicated in the legend. TG/TG: homozygous transgenic. WT/WT: wild-type. TG/WT: hemizygous transgenic. WT control: C57BL/6N. Neg. control: no template control.