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. 2017 Feb 21;45(8):4929–4943. doi: 10.1093/nar/gkx100

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© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 2. — Characterization of non-B DNA structure formation by native PAGE and CD spectroscopy. Native gel electrophoresis results showing folded mobilities under (A) H-DNA/triplex-forming and (C) G4-DNA-forming conditions. R1, R2 = 36-mer; R2-3΄, R2-5΄ = 46-mer; M10 = 10-bp marker; T30/T40 = single-strand polydT markers; DS12/DS24 = double-strand markers. Circular dichroism (CD) spectroscopy results showing (B) a negative peak in the 210–220 nm region (oval) indicates triplex formation under triplex-forming conditions. (D) A negative peak at 240 nm and positive peak at 260 nm suggest parallel G4-DNA formation under G4-DNA-forming conditions.