Figure 1.

Serum TH Levels, bw, and Daily Food Intake in Adult Hypothyroid and Euthyroid Mice. WT, Trhr1 ko (R1 ko), TH-treated Trhr1 ko (R1 ko+TH), athyroid Pax8 ko mice, and WT animals rendered hypothyroid by MMI/perchlorate treatment were analyzed with respect to their serum TH levels (A), bw and daily food intake (B) at the age of 3–4 months. Due to the different genetic background, Pax8 WT animals (mixed C57Bl6/NMRI background) are heavier and exhibit a higher fat content than WT animals on either C57Bl6N (R1-WT) or C57Bl6J background (WT). Body weight was significant lower in hypothyroid animals when compared with background-matched control mice. Daily food intake normalized to bw was not altered. C, Visceral and sc white fat mass were determined with a micro-CT scanner and normalized to total bw. n = 8; *P < .05; **P < .01; ***P < .001.