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. Author manuscript; available in PMC: 2018 May 1.
Published in final edited form as: Dev Biol. 2017 Mar 14;425(1):85–99. doi: 10.1016/j.ydbio.2017.03.011

Figure 1. Two transcriptional variants of Annexin A6 are expressed in the chick head and trigeminal ganglia and are depleted with a translation-blocking morpholino.

Figure 1

(A) Diagram of the two transcriptional variants of Annexin A6 identified through 5′ and 3′ RACE on RNA extracted from both HH17 heads and excised trigeminal ganglia. Transcriptional variant 1 contains a unique first exon (exon 1A) with solely 5′ UTR sequence that aligns with a confirmed NCBI sequence (accession NM_204730.1) but possesses an additional 21 nucleotides. Variant 2 contains an alternate 5′ UTR first exon (exon 1B) that aligns with two predicted NCBI variant sequences. Both variants share a conserved exon 2 splice acceptor site (exon 2 in bold), open reading frame, and 3′ UTR. (B) A translation-blocking Annexin A6 MO was designed to target the conserved start codon of both variants in (A). This MO shares 44% or less similarity with other putative Annexin target sites (only conserved nucleotides are shown) and also does not target other genomic sequences (not shown). (C) Knockdown efficiency of the Annexin A6 MO was assessed as previously described (Wu and Taneyhill, 2012). Immunoblot analysis reveals a 34% reduction in Annexin A6 protein as compared with the control MO-treated lysate.