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The erb1Δ161–200 and erb1Δ201–245 mutants are defective in processing of 27SB pre-rRNA. Total RNA was extracted from GAL-ERB1 strains carrying plasmids containing no insert (pRS314), wildtype ERB1 or erb1 mutant alleles grown in C-Trp+Gal media and shifted to C-Trp+Glu, and assayed by primer extension. Amounts of U2 snRNA assayed in the same primer extension reaction were used as the loading control.
