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Removal of the ITS2 spacer is blocked in the YTM1-C mutant. (A) Pre-rRNA processing in the dominant negative mutant YTM1-C was assayed by northern blotting. U2 snRNA was used as the loading control. The protein composition of preribosomes purified from the YTM1-C mutant using TAP-tagged AF Nop7 as bait was assayed by SDS-PAGE (B), and western blotting (C).
