Skip to main content
. 2017 Jan 23;45(8):4359–4369. doi: 10.1093/nar/gkw1367

Figure 3.

Figure 3.

Effect of PRMT5 on IRES-dependent translation. (A) Schematic representation of bicistronic reporter constructs with different IRESes. (B) MCF-7 Ctrl and MCF-7 P5-KD cells were transfected with indicated IRES-dependent reporters, respectively. The firefly and renilla luciferase activities were measured and the ratios of firefly luciferase activity over renilla luciferase activity were calculated. (C) MCF-7 cells were treated with DMSO or 5 μM EPZ015666 for 5 days and then transfected with indicated IRES-dependent reporters for 24 hours. Cells were then lysed to determine the firefly and renilla luciferase activities. The ratios of firefly luciferase activity over renilla luciferase activity were calculated.