Figure 6.

MDS mutations interact genetically with a Prp2 mutation. (A) Cartoon schematic of Prp2-dependent activation of the spliceosome. Prp2 is believed to destabilize Hsh155 as well as the rest of the SF3b complex from interacting with the BS. The PRP2^Q548N allele likely stalls this process at low temperatures (34). (B) Representative temperature sensitivity growth assays of the given Hsh155 variants in combination with Prp2^WT or Prp2^Q548N when plated on YPD at the given temperatures. Hsh155^K335E partially suppresses Prp2^Q548N and Hsh155^D450G enhances cold sensitivity.