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. 2017 Feb 13;45(8):4564–4576. doi: 10.1093/nar/gkx107

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© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 4. — The livers of Sprtn^H/H mice exhibit increased accumulation of Top1ccs. (A) Expression levels of nuclear Top1 in the indicated tissues of 10-month-old female Sprtn^+/+ mice as assessed by anti-Top1 Western blotting. Histone H1 is shown as a loading control. (B) Immunohistochemistry of Top1ccs in the liver of 4-month-old mice. Cryosections of the liver from Sprtn^+/+ and Sprtn^H/H mice were stained with anti-Top1cc. DNA was stained with Hoechst to visualize nuclei. (C) Quantitation of cells containing Top1cc foci in the indicated tissues of 4-month-old mice. At least 100 cells were scored for Top1cc foci in Sprtn^+/+ and Sprtn^H/H mice. Percentages of cells with 10 or more foci are shown. Values are mean ± s.e.m. (n = 3). No cells were scored as positive for Top1cc foci in the thymus, spleen and kidney. (D) Immunohistochemistry of γH2AX in the liver of 4-month-old mice. Cryosections of the liver from Sprtn^+/+ and Sprtn^H/H mice were stained with anti-γH2AX. DNA was stained with Hoechst to visualize nuclei. (E) Quantitation of cells containing γH2AX foci in the indicated tissues of 4-month-old mice. At least 100 cells were scored for γH2AX foci and percentages of cells with 10 or more foci are shown. Values are mean ± s.e.m. (n = 3). No cells were scored as positive for γH2AX foci in the spleen. *P < 0.05; **P < 0.01 (two-tailed unpaired t-test).