Fig. 5.

2-Photon in vivo voltage imaging of sensory processing under sedation. (a) Example of in vivo cortical regions from a transgenic mouse expressing chimeric VSFP Butterfly in all pyramidal neurons. A single layer II/III pyramidal neuron is outlined in the ROI. (b) Single-trial in vivo voltage responses to auditory stimuli from the pyramidal neuron in the identified ROI in (a) (right), where different auditory stimuli can elicit either depolarizing response (left) or hyperpolarizing response (right). (c) Example of average voltage responses to visual stimulation (drifting gratings of different orientations; number of trials = 10). These data are unpublished examples generated using the methods described in Akemann et al.⁸¹