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. 2017 Jan 20;45(8):e60. doi: 10.1093/nar/gkw1325

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© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 4. — Evaluation of predicted off-targeting potential measures with siRNA-specific differential expression data from 63 siRNA transfection experiments. Pearson's r is given for each correlation analysis between method-specific off-targeting potential measure, RIsearch2-based or siSPOTR-based , and threshold-specific expression-based off-targeting potential measure of 63 siRNAs. The expression-based measure corresponds to the number of transcripts that have a complementary heptamer to the siRNA seed region in their 3΄ UTR and are differentially expressed upon siRNA transfection with a log₂FC value lower than the threshold given on the x-axis. Only significant correlations (P-value < 0.05) are highlighted and the log₂FC threshold −0.3, which is the evaluation threshold employed in the siSPOTR study, is shown with a dashed line.

Inline graphic — Evaluation of predicted off-targeting potential measures with siRNA-specific differential expression data from 63 siRNA transfection experiments. Pearson's r is given for each correlation analysis between method-specific off-targeting potential measure, RIsearch2-based or siSPOTR-based , and threshold-specific expression-based off-targeting potential measure of 63 siRNAs. The expression-based measure corresponds to the number of transcripts that have a complementary heptamer to the siRNA seed region in their 3΄ UTR and are differentially expressed upon siRNA transfection with a log₂FC value lower than the threshold given on the x-axis. Only significant correlations (P-value < 0.05) are highlighted and the log₂FC threshold −0.3, which is the evaluation threshold employed in the siSPOTR study, is shown with a dashed line.