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. 2017 Jan 25;45(8):4401–4412. doi: 10.1093/nar/gkx022

Figure 1.

Figure 1.

Prolonged miR-26a over-expression leads to formation of large bi/mononucleated cells with an increased number of micronuclei. (A) Phase-contrast images of MCF-7 (top panel) and MDA-MB-231 (bottom panel) cells upon transfection with miR-26a and miR-n.c. precursors (5 nM) for 9 days. Scale bar: 100 μm. Arrows indicate multinucleated cells. (B) From left to right: DAPI staining of DNA, Alexa Fluor 488 phalloidin (1:500) staining of F-actin and overlay of the two images of MCF-7 (top panel) or MDA-MB-231 cells (bottom panel) transfected with miR-26a and miR-n.c. precursors (5nM) for 9 days. White arrows indicate aberrant nuclei; red arrows indicate cortical microspikes. Scale bar: 20 μm. The graphs show the percentages of multinucleated cells observed in the two BC cell lines after long term-transfection with the indicated miRNA precursors. 50–100 cells were counted per condition in three independent experiments. Data are mean ± S.E.M. (C) The average size of the nucleus in cells transfected with indicated precursors for 9 days was calculated using ImageJ software; 100 cells were calculated per condition. (D) The number of micronuclei in miR-26a and miR-n.c. transfected cells was calculated following DAPI staining and image acquisition using a confocal microscope. >100 cells were analysed per condition. n = 3.