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. 2017 Jan 11;45(8):e59. doi: 10.1093/nar/gkw1324

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© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 3. — Enriched RCPs at high concentration can be resolved via confocal microscopy. (A) 3D rendered confocal Z stack of 1 pM RCPs labelled with either Cy3 or FITC, and enriched on a NC membrane. (B) A detailed 3D rendered region inside the membrane with Cy3 and FITC labelled RCPs. (C) Co-localization measurement of enriched RCPs and expected numbers of co-localization by chance. Artificial images with objects with random distributions and the same number, size and intensity of the measured RCPs were generated and co-localization were measured with the same parameters (Error bars ±1 st. dev. of the mean, n = 5 for random images, n = 3 for measured RCP images). (D) Distribution of RCP localization inside the membrane as a function of different flow rates used during enrichment.