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. 2017 Feb 28;45(8):4972–4983. doi: 10.1093/nar/gkx138

Figure 6.

Figure 6.

Model of the VcHigB2 toxin bound to the ribosome. The model was obtained by the superposition of the VcHigB2 toxin on EcRelE in the EcRelE–ribosome complex (PDB ID: 4V7J). (A) VcHigB2 positively charged β2-β3 loop (highlighted in yellow) interacts with helies 18 and 34 from the 16S RNA. The conformation of the β2-β3 loop was optimized using ModLoop web server (61). (B) VcHigB2 toxin with bound VcHigA23-33 peptide placed in the VcHigB2 binding site of ribosome using the EcRelE–ribosome complex (PDB ID: 4V7J) as a guide. No obvious clashes are observed between the ribosome or the mRNA substrate and VcHigB2 or VcHigA23-33.