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. 2017 Jan 11;45(8):4782–4795. doi: 10.1093/nar/gkw1343

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© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 6. — RNase III prevents accumulation of the active AapA1_Tr1 mRNA. Expression of AapA1 and IsoA1 RNAs in wild type and isogenic Δrnc H. pylori strains (X47-2AL, 26695 and B128) were analyzed by Northern blot. The aapA1/IsoA1 locus of the 26695 strain (AapA1²⁶⁶⁹⁵) or of the B128 strain (AapA1^B128) was inserted in the X47-2AL strain and its Δrnc derivative. Samples from each strain were collected when culture reached OD₆₀₀ ≈ 1. tRNA Val (t34) served as a loading control and was probed with the FD499 radiolabeled oligonucleotide. Upper panel: AapA1 transcripts revealed with FD47 probe; middle panel: IsoA1 transcripts revealed with FD198 probe.