Table 2.
Insecticidal activity of Cry1Ab, Vip3Aa20 and Cry1F against ECB at 120 h after treatment
| Test protein | Protein conc. (ng/ml) | % Mortality (observed)a | % Mortality (expected) |
|---|---|---|---|
| Cry1Ab | 200 | 66.7 | N/A |
| 100 | 43.1 | ||
| 50 | 45.1 | ||
| 25 | 13.9 | ||
| 12.5 | 5.6 | ||
| Vip3Aa20 | 100 | 0.0 | N/A |
| 50 | 2.8 | ||
| 25 | 7.0 | ||
| 12.5 | 0.0 | ||
| 6.3 | 2.8 | ||
| Cry1F | 400 | 62.5 | N/A |
| 200 | 37.5 | ||
| 100 | 6.9 | ||
| 50 | 1.4 | ||
| 25 | 4.2 | ||
| Test mixtureb | A | 80.0 | 87.5 |
| A/2 | 85.7 | 65.4 | |
| A/4 | 52.8 | 52.5 | |
| A/8 | 22.1 | 15.1 | |
| A/16 | 5.7 | 12.0 |
a
All data were corrected for buffer control mortality using Abbott’s formula.
b
The test mixture A was composed of 200, 100, and 400 ng/ml diet of Cry1Ab, Vip3Aa20, and Cry1F, respectively, as described in the Methods section.