Fig. 4. Synthesis of tiDELs based on β-carbolines, and pyrazol(in)es. Reagents and conditions: (a) 2% TFA/CH₂Cl₂, room temperature, overnight; (b) NaCNBH₄, 40 °C, overnight; (c) aq. NH₃/MeNH₂, room temperature, 30 min; (d) 10% TFA/CH₂Cl₂, 6 h; (e) A (see Table 2), MeCN, 50 °C, overnight; (f) B (see Table 2), AcOH, 60 °C, overnight; (g) T4 ligase, room temperature, overnight; (h) EDC, HOAt, DEAE sepharose, room temperature, overnight. R: p-aminocarbonylphenyl; wavy bond to hexT denotes 5′-amino-PEG(4)-linker.