Skip to main content
. 2017 Mar 1;8(5):3390–3398. doi: 10.1039/c7sc00441a

Fig. 1. Characterizations of NIR800 Pdots and specific cell labeling by NIR800 Pdots. (a) Hydrodynamic diameter of the NIR800 Pdots. The inset shows a representative TEM image. The scale bar represents 100 nm. (b) Spectral character of the NIR800 Pdots. (c) Flow cytometry of MCF-7 cells labeled with NIR800 Pdots or PE-Cy7 dye via specific antigen–antibody and streptavidin–biotin interaction with nonspecific labeled cells as control. (d) Fluorescence imaging of cell-surface marker (EpCAM) in MCF-7 cells labeled with Pdot–streptavidin. The scale bar represents 20 μm. (e) Imaging of microtubules in MCF-7 cells labeled with Pdot–streptavidin. In d and e, the right panels show MCF-7 cells incubated sequentially with primary antibody and Pdot–streptavidin; the left panels show control samples in which the cells were incubated with Pdot–streptavidin alone (no primary antibody). The scale bar represents 20 μm.

Fig. 1