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. 2012 Nov 2;27(1):63–73. doi: 10.1210/me.2012-1256

Fig. 2.

Fig. 2.

Disappearance of EGFP expression from indifferent gonads through a mutation at the Lhx9-binding site of the Ad4BP/SF-1 gene promoter. A, Two DNA constructs, SmAc-1.8-Ad4BP-EGFP and SmAc-1.8-Ad4BP(LBmut)-EGFP, were used for transgenic mouse studies. SmAc-1.8-Ad4BP-EGFP consists of a 1.8-kb fetal Leydig cell-specific enhancer (SmAc-1.8); the Ad4BP/SF-1 gene promoter contains a 5.8-kb region upstream from the initiator methionine in the second exon (Ad4BP/SF-1 gene promoter). EGFP is the reporter gene, and poly(A) signal is represented as pA. The DNA composition of SmAc-1.8-Ad4BP(LBmut)-EGFP is the same as SmAc-1.8-Ad4BP-EGFP, except that the LHX9-binding site in the Ad4BP/SF-1 gene promoter is mutated (indicated by X). B, EGFP expression in the testes of the transgenic mice carrying SmAc-1.8-Ad4BP-EGFP (upper panels) and SmAc-1.8-Ad4BP(LBmut)-EGFP (lower panels) at E10.5, E12.5, and E18.5 fetuses is shown. Tes, Testis.