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Expression of EGFP in the testes of the two lines of transgenic mice. Gonads were prepared from the transgenic mice carrying SmAc-1.8-Ad4BP-EGFP (upper panels) and SmAc-1.8-Ad4BP(LBmut)-EGFP (lower panels) at E11.5, E12.5, and E18.5. They were subjected to immunofluorescence analyses by using the combination of the antibodies to EGFP (green) and Ad4BP/SF-1 (red) or EGFP (green) and 3β-HSD (red). 4′6′-Diamidino-2-phenylindole (blue) was used to stain nuclei. Bars, 50 μm.
