Fig. 5.

Differential gene expression among fetal Leydig, adult Leydig, and fetal Sertoli cells. A, Fetal Sertoli cells (FSCs) and fetal Leydig cells (FLCs) were isolated from E18.5 testes and adult Leydig cells (ALCs) were prepared from 9-wk-old male mice. Total RNA was prepared from each cell type and subjected to quantitative RT-PCR for the genes indicated. The amount of mRNA relative to Actb (encoding β-actin) is plotted. Values are the means ± sd. B, The expression of HSD17B3 was examined in the fetal and adult testes. Sections of fetal (E18.5) and adult testes (P56) were used for immunohistochemical staining using rat monoclonal antibody for mouse HSD17B3. Bars, 100 μm.