. 2012 Nov 2;27(1):63–73. doi: 10.1210/me.2012-1256

Table 1.

Primers used in quantitative RT-PCR analyses

Gene name	Primer (forward/reverse)	Primer sequence (5′–3′)
Actb (β-actin)	Forward	`AGGGTGTGATGGTGGGAATGG`
	Reverse	`TGGCTGGGGTGTTGAAGGTCT`
Nr5a1 (Ad4BP/SF-1)	Forward	`AAGCCACTCTGTAGGACCAAGC`
	Reverse	`TGTAAATCTGACGCGAAAGCAG`
Sox9	Forward	`CCTTGAGCCTTAAATCCCGGCTGC`
	Reverse	`CTTTGCTTGCCCCTGGCTTTCCTT`
Amh (Mis)	Forward	`GAACCTCTGCCCTACTCGGG`
	Reverse	`AAGTCCACGGTTAGCACCAAA`
Hsd3b1	Forward	`CAAGTGTGCCAGCCTTCATCT`
	Reverse	`TTCATGATTCTGTTCCTCGTGG`
Hsd3b6^a	Forward	`TTTTTTTGAGGTATTGACAAGTATTTATTG`
	Reverse	`TCCCCATTCAGAGCATGTATAGC`
Cyp11a1 (P450SCC)	Forward	`CGAATCGTCCTAAACCAAGAG`
	Reverse	`CACTGATGACCCCTGAGAAAT`
StAR	Forward	`TACATCCAGCAGGGAGAGGTG`
	Reverse	`CAGCGCACGCTCACGAAGTCT`
Cyp17a1	Forward	`GGGTGACCCCAAGGTGGTGGTCTTTCT`
	Reverse	`TGGGATCCGGGACGTTAGATTCGG`
Hsd17b3	Forward	`ATGGAGTCAAGGAGGAAAGGC`
	Reverse	`GGCTGTAAAGAGGCCAGGG`
Ddx4 (Mvh, VASA)	Forward	`GCACACGTTGAATACAGCGGGGAT`
	Reverse	`TGGGAGGAAGAACAGAAGAACAGG`
Pdgfra	Forward	`CAAACCCTGAGACCACAATGG`
	Reverse	`TGATGCCCACATAGCCTTCAT`
Maf (c-Maf)	Forward	`CCGTTGTACATGCACTGAAGGCGT`
	Reverse	`TGCCCCAAAGCAGGCATCATTGTT`
Mafb	Forward	`CCGCGAGGCTTATTCCAAG`
	Reverse	`AGCCAGAAGTGTCCAGACGG`

Gene names (and aliases), direction of the primers (forward or reverse), and primer sequences are shown.

^{^a}

Primers for Hsd3b6 are originally designed by O'Shaughnessy et al. (38).