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9-Cis-RA enforces a dissociation of the core RFC complex (hRFC2/4/5) and association of hRFC3 with hRXRα. Expression vectors for five HA-hRFC family members were cotransfected with hRXRα expression vector in 293T cells. The cells were incubated for 24 h with 1 μm 9-cis-RA (+) or the same volume of DMSO (−). Immune complexes were analyzed as described in Fig. 4. IP, Immunoprecipitation; WB, Western blot.
